The effect of clearing and staining technique differentially affects morphometric and allometric analysis in fixed Chloroscombrus chrysurus (Perciformes: Carangidae) species.

Background. In morphometric and allometric analysis, the degree of variation attributable to non-biological causes is ignored. The effects this on subsequent statistical analyzes are unknown. It unclear whether sample conservation induces substantial in shape such affects inference interpretation. Therefore, fish skeletal studies, routinely prepared for osteological studies using a common procedure known as clearing staining, but samples frequently measured after process. various it has been determined that fixation fishes produces deformation, with decrease size, effect not evaluated process clearing-staining analysis Objective. Determine staining analyses. Methods. Thirty-three specimens species Chloroscombrus chrysurus (Linnaeus, 1766) previously fixed within 10% formalin. These were before alizarin red S staining. Three applied: deformation analysis. Only statistically significant results used. Results. was found affected 90% indices, well 90.9% measurements, where 54.5% decreased 36.4% increased. With respect 80% length-length relationships remain same. Conclusions. C. chrysurus’s morphological variation, by variables methods